Genetically Certified Oocyte® technology
Ovogene is the only one egg bank in the world offering the unique service of Genetically Certified Oocyte® technology, this includes:
Oocyte NGS Screening- determines aneuploidy of oocytes via first polar body biopsy.
100% euploid oocytes.
Higher blastocyst rates
Genetically Certified Oocyte® technology warrants a higher chance of getting pregnant from the 1st attempt*, avoiding numerous attempts.
Genetic laboratory certification.
*Numerous factors can influence pregnancy success rates- egg quality, sperm quality, experience of embryologist, patient's health conditions, lab equipment. We guarantee that the quality of eggs is appropriate to achieve a successful pregnancy, but we can not take responsibility for other independent factors.
What is Certified oocyte?
- They are genetically screened eggs that have been found to have a normal chromosomal constitution. This can reassure individuals using donor eggs that the oocytes will be of better quality and therefore have a better chance of a successful pregnancy.
What does oocyte genetic error mean and how is it detected?
- Genetic errors in the oocyte occur as a result of meiotic malsegregation during oogenesis. Polar body analysis is an important method in detecting these errors.
- Female meiotic errors are the main cause of whole chromosomal aneuploidy in human embryos and significantly contribute to early embryo loss.
Why is it important to genetically screen the oocyte?
- The genetic structure of an oocyte directly affects pregnancy success.
- Aneuploidy embryos reduce pregnancy success or can lead to births with chromosomal abnormalities such as Down syndrome.
- Analysis of the polar body revealed that on average a third of the eggs from young females had genetic errors (Gianaroli et al., 2009).
What are the advantages of oocyte NGS testing?
- Considering the problem of mosaicism, which is the major limitation of the embryo testing, the direct testing of oocyte by polar body analysis have a practical value in improving accuracy and reliability of selecting euploid embryos for transfer (Anver Kuliev, 2011).
- Despite limitations of PB testing in which only maternally derived errors are identified, the PB testing still provides detection and avoidance of the transfer of the majority of aneuploid embryos, as approximately 95% of chromosomal errors originate from female meiosis alone (Anver Kuliev, 2011).
What about survival rates and IVF outcomes?
According to current research and statistics, almost 20-30% of donor eggs are aneuploidy (J. Horne, 2016).
Oocyte NGS Aneuploidy testing is unique, as it promises to differentiate between aneuploid and euploid oocytes. The aneuploidy testing aims to optimise the egg donation programme by only selecting the best quality, euploid oocytes.
Euploid oocytes increase the probability of a euploid embryo, hence offer better survival rates. Oocyte testing will avoid the need for NGS embryo testing, therefore, patients can avoid the traumatic process of embryo biopsy, freezing and re-thawing again. No difference in survival rate has been identified among vitrified donor oocytes which have undergone biopsy and oocytes that have not.
Ovogene Egg Bank is able to offer better clinical outcomes for egg donation, with the use of euploid oocytes only. By avoiding using aneuploid oocytes, we can offer a more cost effective egg donation programme.
Obligatory tests, before purchasing programs with NGS screening :
- *FISH sperm analysis, Karyotype
- *Endometrium thickness receptivity
- *Results of histeroscopy
- *Sperm factors
- Indicators of the spermogram - more than 1 million sp in ml
- More than 1% are morphologically normal
- Fr DNA within the norms of values
- FISH sperm analysis
- Karyotype - husband
- The patient is up to 50 (55-?) Years old
- Normal p-li hysteroscopies during the year
- The minimum thickness for the 8th - 12th day of the cycle is 8 mm
- Normal structure
- In the absence of hystero - ultrasound and histology
- Absence of serious autoimmune and general diseases such as Diabetes, hypertension, c \ c
Polar Body Biopsy and Aneuploidy testing
Blastomere testing for aneuploidy has an important limitation of extremely high rates of mosaicism at the cleavage stage. Most of the time, especially for egg donation programmes, blastocyst trophectoderm NGS testing is not very useful as the number of blastocysts are limited.
Polar body biopsy is a sensitive process that requires intricate manipulation. For effective handling, a wealth of practical experience and theoretical knowledge is essential. Our scientific team has an abundance of expertise within polar body biopsy and oocyte aneuploidy testing with NGS. It is extremely important to have a strong and efficient vitrification (cryopreservation) process for the evaluated oocytes. A special vitrification protocol for polar body biopsied oocytes was designed by our dedicated team to guarantee high survival rates after the thawing process.
After polar body biopsy, we analyse the genetic aptitude of oocytes by NGS screening. Approximately 20% of high quality morphological donor oocytes exhibit aneuploidy. Our ambition is to supply only euploid oocytes to our customers, thus increasing patient’s chances of a clinical pregnancy. Euploid oocytes indicate a strong potential for the fertilization, a high quality of euploid blastocysts and non-fragmented embryo development. Our customer have a unique opportunity to get only euploid oocytes, excluding aneuploid oocytes, for their egg donation programme.
Meiotic spindle determination before oocyte vitrification.
The rates of oocyte having normal spindle and chromosomal configuration is 75%-80%. Still approximately 20% oocytes has abnormal meiotic spindle or meiotic spindle take place on critical area which may create a damage during fertilization process by ICSI method. Ovogene Egg Bank is freezing only normal meiotic spindle view oocytes with special software and also customer will have chance to get real time picture and location of meiotic spindle . By this way you will have chance to avoid risk of meiotic spindle damage during sperm injection by ICSI method.
Oocytes Freezing quality.
There is a way with time lapse camera microscopy to show actual quality of vitrification! Time lapse camera microscopy will create real time quality with HD module and they will be able to see oocyte activation in the vitrification and equilibration solution during cryopreservation. Ovogene Egg Bank aim to work with maximum transparency with customer. Our customer will be able to join each process of donor egg vitrification by this way. Our customer will be sure about our morphological selection criteria, vitrification skills of embryologist and high quality of vitrification process as well.