Genetically certified oocyte technology | OVOGENE Donor Bank

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Ovogene is the only bank in the world offering the unique service of Genetically Certified Oocyte® technology, which provides:

  • oocyte NGS screening — determines aneuploidy of oocytes via first polar body biopsy
  • 100% euploid oocytes
  • higher blastocyst rates
  • warranty of a higher chance of getting pregnant from the 1st attempt*, hence avoiding numerous attempts
  • genetic laboratory certification.

*Numerous factors can influence pregnancy success rates, including egg quality, sperm quality, the experience of the embryologist, patient's health conditions, and lab equipment. We guarantee that the quality of eggs is appropriate to achieve a successful pregnancy, but we cannot take responsibility for other independent factors.

What is a certified oocyte?

It is a genetically screened egg that has been found to have a normal chromosomal constitution. This can reassure individuals using donor eggs that the oocytes will be of better quality and therefore have a higher chance of a successful pregnancy.

What does oocyte genetic error mean and how is it detected?

  • Genetic errors in the oocyte occur as a result of meiotic malsegregation during oogenesis. Polar body analysis is a method of detecting these errors.
  • Female meiotic errors are the main cause of whole chromosomal aneuploidy in human embryos and significantly contribute to early embryo loss.

Why is it important to genetically screen the oocyte?

  • The genetic structure of an oocyte directly affects pregnancy success.
  • Aneuploidy embryos reduce pregnancy success or can lead to births with chromosomal abnormalities, such as Down syndrome.
  • Analysis of the polar body revealed that, on average, a third of the eggs from young females had genetic errors (Gianaroli et al., 2009).

What are the advantages of oocyte NGS testing?

  • Themain advantages of using polar bodybiopsy is that there would be less discarded embryos, which would be moreethical and less traumatizing for many patients.
  • Firstand second polar bodies may not be required for further embryonic development,hence viability of the oocyte is not affected and resulting embryos arepreserved.

Considering the problem of mosaicism, which is a major limitation of embryo testing, the direct testing of oocytes bipolar body analysis has practical value in improving the accuracy and reliability of selecting euploid embryos for transfer (Kuliev, 2011).

A constraint of polar body testing is that only maternally derived errors are identified. But it has been shown that approximately 95% of chromosomal errors originate from female meiosis alone (Kuliev, 2011). Despite the limitations, polar body testing still provides detection of chromosomal irregularities, and therefore, the majority of aneuploid embryos can be avoided for transfer.

What about survival rates and IVF outcomes?

According to current research and statistics, almost 20-30% of donor eggs are aneuploidy (Horne, 2016).

Oocyte NGS aneuploidy testing is unique as it promises to differentiate between aneuploid and euploid oocytes. The aneuploidy testing aims to optimize the egg donation program by only selecting the best quality euploid oocytes.

Euploid oocytes increase the probability of a euploid embryo, hence offering better survival rates. Oocyte testing will avoid the need for NGS embryo testing, therefore, patients can avoid the traumatic process of embryo biopsy, freezing and re-thawing again. No difference in survival rate has been identified among vitrified donor oocytes which have undergone biopsy and oocytes that have not.

Ovogene is able to offer better clinical outcomes for egg donation with the use of euploid oocytes only. By avoiding using aneuploid oocytes, we can offer a more cost-effective egg donation program.

What are the advantages of oocyte NGS testing?

  • *FISH sperm analysis, Karyotype
  • *Endometrium thickness receptivity
  • *Results of hysteroscopy
  • *Sperm factors
  • Indicators of the spermogram - more than 1 million sp in ml
  • More than 1% are morphologically normal
  • Fr DNA within the norms of values
  • FISH sperm analysis
  • Karyotype - husband
  • The patient is up to 50 (55-?) years old
  • Normal p-li hysteroscopies during the year
  • The minimum thickness for the 8th - 12th days of the cycle is 8 mm
  • Normal structure
  • In the absence of hystero - ultrasound and histology
  • Absence of serious autoimmune and general diseases such as diabetes, hypertension, etc.

Polar Body Biopsy and Aneuploidy testing

Blastomere testing for aneuploidy has an important limitation of extremely high rates of mosaicism at the cleavage stage. Most of the time, especially for egg donation programs, blastocyst trophectoderm NGS testing is not very useful as the number of blastocysts is limited.

Polar body biopsy is a sensitive process that requires intricate manipulation. For effective handling, a wealth of practical experience and theoretical knowledge is essential. Our scientific team has an abundance of expertise in polar body biopsy and oocyte aneuploidy testing with NGS. It is extremely important to have strong and efficient vitrification (cryopreservation) process for the evaluated oocytes. Our dedicated team designed a special vitrification protocol for polar body biopsied oocytes to guarantee high survival rates after the thawing process.

After polar body biopsy, we analyze the genetic aptitude of oocytes by NGS screening. Approximately 20% of high-quality morphological donor oocytes exhibit aneuploidy. Our ambition is to supply only euploid oocytes to our customers, thus increasing a patient’s chances of clinical pregnancy. Euploid oocytes indicate a strong potential for fertilization, high quality of euploid blastocysts and non-fragmented embryo development. Our customers have a unique opportunity to get only euploid oocytes, excluding aneuploid oocytes, for their egg donation program.

Meiotic spindle determination before oocyte vitrification.

The probability of an oocyte having a normal spindle and chromosomal configuration is 75%-80%. Consequently, approximately 20% of oocytes have an abnormal meiotic spindle, or the meiotic spindle is located in a critical area, which may create damage during the fertilization process, such as ICSI. Ovogene only freezes oocytes with a normal meiotic spindle view, with this confirmed through special software. Also, customers are able to get real-time pictures and the location of the meiotic spindle. This helps avoid the risk of meiotic spindle damage during sperm injection by the ICSI method.

Oocyte freezing quality

With time-lapse camera microscopy, it is possible to show the quality of vitrification. The time-lapse microscopy camera creates real-time, HD-quality images, and you can see oocyte activation in the vitrification and equilibration solution during cryopreservation. Ovogene aims for maximum transparency with our customers. Therefore, our client will be able to understand each process of donor egg vitrification. This provides assurance to our customers about our morphological selection criteria, the vitrification techniques of our embryologists and our well-constructed process of vitrification.

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